However, this weekend saw a much stronger showing from the other films than last week, with 'Rio 2,' a animated musical starring the voices of Anne Hathaway and Jesse Eisenberg, closely following 'Winter Soldier' but ended up coming just short by earning $39 million.

Rio 2
'Rio 2' came in a close second on its opening weekend

In 3rd place was the low-budget horror flick 'Oculus' raking in $12 million to beat Kevin Costner's new football film 'Draft Day,' which finished with $9.8 million.

Rounding off the top five was the sci-fi epic 'Divergent', adapted from Veronica Roth's novel series, which earned a modest $7.5 million.

The top ten films of this weekend, via Box Office Mojo, are below:

1.Captain America: The Winter Soldier, $41.4 million

2.Rio 2, $39 million

3.Oculus,$12 million

4.Draft Day, $9.8 million

5.Divergent, $7.5 million

6.Noah, $7.5 million

7.God's Not Dead, $5.5 million

8.The Grand Budapest Hotel, $4.1 million

9.Muppets Most Wanted, $2.2 million

10.Mr. Peabody and Sherman, $1.8 million

Captain America
'Captain America: The Winter Soldier' spends another week at the No.1 spot